Determination of Mono-glycerate in Bread by Gas Chromatography

KeyWord:gas chromatography£¨GC£©  bread  mono-glycerate

Abstract:

      A gas chromatographic£¨GC£© method was established for the determination of three kinds of mono-glycerates£¨e.g.glycerate monocaprylin£¬glyceryl monodecanoate and glycery monolaurate£© in bread.The sample was extracted with dichloromethane-n hexane£¨1¡Ã1£©.The extract was concentrated£¬then extracted with acetonitrile saturated with n-hexane£¬and detected by GC with the external standard method.The calibration curves for three mono-glycerates were linear in the range of 20-1 000 mg/L with correlation coefficients more than 0.999.The limits of quantitation of 3 monoglycerides are 12.8-35.2 mg/kg.The spiked recoveries were in the range of 81.1%-95.7% with RSDs of 2.9%-8.8%.The method has the advantages of simple operation£¬high sensitivity£¬good reproducibility and selectivity£¬and could meet the requirements for trace analysis of three monoglyceride in bread.