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Determination of Magnolol,Chlorogenic Acid,Caffeic Acid in Qinggan Lidan Oral by Capillary Electrophoresis with LVSS-Reverse Current Flow Accumulation-CZE |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:large volume sample injection(LVSS) capillary zone electrophoresis(CZE) magnolol chlorogenic acid caffeic acid |
Author | Institution |
张瑞瑞,李利军,杨兰兰,孙科,崔越,崔福海,罗应,李彦青 |
广西工学院生物与化学工程系 |
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Abstract: |
A large volume sample injection(LVSS)-reverse current flow accumulation capillary zone electrophoresis(CZE) method was used for the simultaneous separation and determination of magnolol,chlorogenic acid and caffeic acid in Qinggan Lidan oral.The result indicated that a best separation of three materials were obtained on an uncoated fused silica capillary column(50 cm×50 μm,effective length 36 cm) by using background electrolyte containing 40 mmol/L Na2B4O7,20mmol/L NaH2PO4,pH 9.0).The detection wavelength was set at 220 nm,with the separation voltage at 16 kV,the sample injection voltage at-12 kV and the injection time in 356 s.Under the optimum conditions, the separation of three compounds was performed within 20 min with relative standard deviations(RSDs) of peak area less than 4%.The detection limits(S/N=3) of magnolol,chlorogenic acid and caffeic acid were 184.2,36.07,77.99 ng/L,respectively. |
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