A simple and rapid method was developed for the simultaneous determination of 11 fat-soluble colorants,including Sudan Orange G,Dimethyl Yellow,Para Red,Citrus Red 2,Sudan Red G,SudanⅠ,SudanⅡ,Sudan Ⅲ,Sudan Red 7B,Sudan Red B and Sudan Ⅳ in chilies.The sample was extracted with ethyl acetate and then purified by gel permeation chromatography(GPC).The targeted compounds were detected by HPLC/DAD detector and quantified by the external standard method.The calibration curves of 11 colorants were linear in the range of 20-1 000 μg/L with correlation coefficients more than 0.998 .The average recoveries of 11 colorants at three spiked levels of 10,20,40 μg/kg were between 75.8% and 90.8%,with relative standard deviations of 3.0%-7.8%.The quantitation limits(S/N≥10)were all 10 μg/kg.The method is convenient and accurate,and could be applied in the determination of 11 colorants in chilies and chili sauce. |