Simultaneous Determination of 11 Fat-soluble Colorants in Foodstuffs by HPLC/DAD
  
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DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:gel permeation chromatography(GPC)  high performance liquid chromatography(HPLC)  diode array detection(DAD)  colorants  chilies
  
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马育松,李玮,艾连峰,郭春海,陈瑞春 河北出入境检验检疫局
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Abstract:
      A simple and rapid method was developed for the simultaneous determination of 11 fat-soluble colorants,including Sudan Orange G,Dimethyl Yellow,Para Red,Citrus Red 2,Sudan Red G,SudanⅠ,SudanⅡ,Sudan Ⅲ,Sudan Red 7B,Sudan Red B and Sudan Ⅳ in chilies.The sample was extracted with ethyl acetate and then purified by gel permeation chromatography(GPC).The targeted compounds were detected by HPLC/DAD detector and quantified by the external standard method.The calibration curves of 11 colorants were linear in the range of 20-1 000 μg/L with correlation coefficients more than 0.998 .The average recoveries of 11 colorants at three spiked levels of 10,20,40 μg/kg were between 75.8% and 90.8%,with relative standard deviations of 3.0%-7.8%.The quantitation limits(S/N≥10)were all 10 μg/kg.The method is convenient and accurate,and could be applied in the determination of 11 colorants in chilies and chili sauce.
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