Chemical Components Identification and Fingerprint Analysis of Bansha Herbal Tea Using High Performance Liquid Chromatography Coupled with Mass Spectrometry

KeyWord:Bansha herbal tea  chemical constituents  fingerprint  quality control  high performance liquid chromatography coupled with mass spectrometry

Abstract:

      A high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF-MS) and high performance liquid chromatography ion trap mass spectrometric(HPLC-IT-MS) method were used for the identification of constituents of ¡°Huangzhenlong(HZL)¡± and ¡°Pingantang(PAT)¡± Bansha herbal tea(BHT).The results showed that HZL and PAT BHT contained 40 major chemical constituents.By comparing the mass spectrometric data of each constituent with those of literature,17 of the 40 constituents were identified.Among them,13 were confirmed by the comparison of their retention time and MS data with those of reference substances.In addition,chemical fingerprints of HZL and PAT BHT were established by HPLC-IT-MS,and principal component analysis(PCA) was performed to evaluate the quality consistency and stability of 41 batches of BHT samples.PCA results showed that HZL and PAT BHT samples were significantly different,indicating their formulas and the production processes were different.The developed fingerprinting method was fast,efficient and robust for the quality control of BHT products.