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Determination of Benzoquanmine in Liquid Products of Milk by LC-MS/MS |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:benzoquanmine high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS) milk |
Author | Institution |
郝成婷,王红松*,徐炎,周志荣,陈智栋 |
1.常州大学石油化工学院;2.常州出入境检验检疫局 |
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Abstract: |
A method was developed for the determination of benzoquanmine in liquid products of milk by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The conditions of extraction and parameters of LC-MS were optimized.The sample was enriched and purified with an LC-C18 SPE cartridge,then separated on a ZORBAX Eclipse Plus C18 column using a mixture of acetonitrile-water(15∶85) as mobile phase at a flow rate of 0.3 mL/min.The detection of benzoquanmine was performed by mass spectrometry(ESI-MS) under positive-ion electrospray ionization mode.The two precursor-product ion pairs,m/z 104.1 and m/z 146.0,were used as the quantitative and qualificative ions,respectively.The external standard method was used for quantitative analysis.The results showed that a good linearity for benzoquanmine existed in the range of 2-100 μg/L with a correlation coefficient of 0.999 1.The limits of detection(LODs) and limits of quantitation(LOQs) were 1.0 μg/kg and 3.0 μg/kg,respectively.The average recoveries of benzoquanmine at three spiked levels were in the range of 77%-105%,with relative standard deviations(RSDs) of 2.3%-5.0%.The proposed method was simple and accurate,and was suitable for the determination of benzoquanmine in liquid products of milk. |
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