Simultaneous Separation of Tetracyclines and Penicillins by Micelle Capillary Electrophoresis

KeyWord:micelle capillary electrophoresis(MEKC)  simultaneous separation  tetracyclines(TCs)  penicillins(PENs)

Abstract:

      A method for the simultaneous separation of seven kinds of drugs including tetracyclines£¨TCs£© and penicillins£¨PENs£© by micelle capillary electrophoresis£¨MEKC£© was developed.Effects of some important factors such as composition£¬pH value and concentration of running buffer£¬surfactant concentration£¬separation voltage and separation temperature were investigated£¬and the factors were optimized by the L16£¨45£© orthogonal test.The optimal conditions were as follows£ºthe running buffer£º40 mmol/L potassium dihydrogen phosphate-20 mmol/L borax solution£¨pH 7.9£©£¬SDS concentration£º65 mmol/L,separation voltage£º28 kV£¬separation temperature£º28 ¡æ£¬UV detection wavelength£º350 nm and 200 nm£®Seven kinds of drugs were baseline separated within 25 min.The calibration curves showed good linearities in the range of 1.56-50 mg/L£¬with correlation coefficients£¨r2£© of 0.997 9-0.999 9£®RSDs£¨n=6£© were in the range of 4.1%-7.3% for peak area and 0.33%-0.67% for migration time.The average recoveries of seven kinds drugs at three spiked concentration levels of 2.0£¬5.0£¬10.0 mg/kg ranged from 83.6% to 93.3%£¬with RSDs£¨n=6£© of 4.7%-7.6%£®The method was rapid£¬simple£¬accurate and high sensitive,and was successfully applied in the determination of seven kinds of drugs in the ponds and lakes of Hefei and its surrounding areas.