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Determination of Phenazine-1-carboxylic Acid Residue in Ginseng by Liquid Chromatography-Tandem Mass Spectrometry |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:liquid chromatography-tandem mass spectrometry(LC-MS/MS) phenazine-1-carboxylic acid ginseng |
Author | Institution |
FU Xin-wei,HUANG Chao-qun,JIANG Qin-ting,ZHU Xiao-yu,CHEN Xiao-mei,MO Wei-min |
1.浙江工业大学化学工程与材料学院;2.浙江省检验检疫科学技术研究院 |
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Abstract: |
A liquid chromatography coupled with electrospray ionization tandem mass spectrometric(LC-MS/MS) method was developed for the determination of phenazine-1-carboxylic acid in ginseng.The ginseng samples were soaked with water,extracted with acetonitrile and cleaned up by dispersive solid phase extraction,separated by chromatography,then detected by MS/MS under multiple reaction monitoring(MRM) mode via positive electrospray ionization.The ion-pair of 225.1/179.1 was used for quantitative analysis,and the ion-pair of 225.1/125.1 was selected for qualitative analysis.The external standard method was used for the quantification analysis.The limit of quantitation(LOQ) of phenazine-1-carboxylic acid was 0.01 mg/kg.The standard calibration curve was linear in the range of 0.5-10 μg/L,with correlation coefficient more than 0.99.The recoveries of phenazine-1-carboxylic acid in ginseng were in the range of 82%-100% at three spiked levels of 0.01,0.02 and 0.10 mg/kg,with relative standard deviations(RSDs) not more than 6.8%.The result showed that the method was simple,rapid and highly sensitive,and could be applied in the determination of phenazine-1-carboxylic acid in ginseng. |
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