Study on Detection of Hg£¨¢ò£© Based on Single Nucleic Acid/AuNPs/Mercury Ion Enzyme Mimetics

KeyWord:Hg2+  detection  gold nanoparticle  mimetic peroxidase

Abstract:

      Mercury£¨Hg2+£© ions deposited on the surface of gold nanoparticles£¨AuNPs£© absorbing single strand DNA£¨ssDNA£© can promote the peroxidase-like activity of AuNPs.Based on this£¬a highly sensitive detection of Hg2+ was developed.Here it was found that ssDNA can promote the activity of AuNPs-Hg2+ mimetic peroxidase due to the adsorption of ssDNA on the AuNPs surface.The catalytic activity of AuNPs-Hg2+ mimetic peroxidase increases with increase of ssDNA concentration.The ssDNA-AuNPs-Hg2+ mimetic peroxidase can be applied in the detection of Hg2+ in a linear range of 10-1 000 nmol/L with a detection limit as low as 3.0 nmol/L under optimized condition.The method was simple£¬rapid£¬low cost and stable£¬and had high application potential to detect Hg2+ in the environment£¬food and other samples.