Performance Study of a New Reversed-phase and Strong Cationic Exchange Solid Phase Extraction Cartridge

KeyWord:new reversed-phase and strong cationic exchange SPE cartridge  adsorption properties  melamine  high performance liquid chromatography tandem mass spectrometry£¨HPLC-MS/MS£©

Abstract:

      In this paper,the adsorption properties of a new reversed-phase and strong cationic exchange solid phase extraction£¨SPE£© cartridge were studied by using high performance liquid chromatography tandem mass spectrometry£¨HPLC-MS/MS£©.The optimum experimental conditions,including pH value,flow rate,the maximum adsorption capacity and the saturated adsorption capacity were investigated.The adsorption kinetics at different initial concentrations,the thermodynamics at different temperatures and the isotherms of melamine on the new SPE sorbent were studied.The optimal conditions were as follows:pH value:5.0,flow rate:1.0 mL/min,elution volume:1.0 mL,the maximum adsorption capacity:900 ¦Ìg.The saturated adsorption capacity at pH 5.0 and 30 ¡æ was 81.45 ¦Ìg/mg.The adsorption kinetic data were well described with pseudo-second-order model and the equilibrium data were well fitted by Langmuir model.The calculated thermodynamic parameters indicated that the adsorption of melamine on the new SPE sorbent was spontaneous and endothermic,and the adsorption was a chemisorption process.