Determination of H2O2 Using Three-dimensionally Ordered Macroporous£¨3DOM£© Gold-nanoparticle-doped Titanium Dioxide£¨GTD£© Film Modified Electrode

KeyWord:three-dimensionally ordered macroporous structure£¨3DOM GTD£©  cytochrome c£¨Cyt c£©  direct electrochemistry  hydrogen peroxide

Abstract:

      The three-dimensionally ordered macroporous gold-nanoparticle-doped titanium dioxide£¨3DOM GTD£© film was modified on the indium-tin oxide£¨ITO£© electrode surface and cytochrome c£¨Cyt c£© was successfully immobilized on the 3DOM GTD/ITO.A kind of Cyt c enzyme biosensor£¨Cyt c/3DOM GTD/ITO£© was prepared.The properties of 3DOM GTD film were characterized by transmission electron microscopy£¨TEM£© and scanning electron microscopy£¨SEM£©.UV-Vis spectrum indicated that the Cyt c immobilized on the film retained its biological activity and the secondary structure of Cyt c was not destroyed.The direct electrochemistry£¨DET£© of Cyt c on the surface of the modified electrode and its electric catalytic behavior to H2O2 were also studied.The results indicated that Cyt c showed an obviously direct electrochemistry response.The peak currents were linear with scan rates,which indicated that the electrochemical behavior of Cyt c/3DOM GTD/ITO electrode was a surface-controlled process.The Cyt c/3DOM GTD/ITO electrode showed a good catalytic performance to hydrogen peroxide,and a wide linear range of 3.0¡Á10-7-1.7¡Á10-5 mol/L was obtained with a detection limit£¨S/N=3£© of 3.6¡Á10-8 mol/L and a rapid response of 5 s.The electrode showed a good reproducibility and stability.