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Determination of Oxytetracycline in Food by Indirect Competitive Enzyme-linked Aptamer Assay |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:oxytetracycline aptamer enzyme-linked aptamer assay(ELAA) food |
Author | Institution |
TANG Zong-gui,LIU Chang-bin,LUO Xiao-ling,KANG Li-chao,SUN Feng-xia,LU Chun-xia* |
1.新疆农垦科学院分析测试中心农业部食品质量监督检验测试中心(石河子);2.新疆农垦科学院畜牧兽医研究所 |
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Abstract: |
An indirect competitive enzyme-linked aptamer assay(ic-ELAA) was developed for monitoring oxytetracycline(OTC) in complex food matrix.Various factors such as OTC-BSA concentration,aptamer concentration,avidin-HRP concentration,coating buffer,blocking agent,and binding buffer were evaluated for the performance of ELAA.The optimized ELAA showed a good sensitivity(IC50 of 6.3 ng/mL) and a wide linear range(0.5-50 ng/mL) for OTC.There was no obvious cross reactivity(CR) with most of the tetracyclines pesticides.Recoveries of OTC in food samples,including milk,milk powder,chicken,fish and honey,ranged from 62.1% to 102% with relative standard deviations(RSDs) less than 15%.In addition,the developed ELAA showed a good correlation with the high performance liquid chromatographic method(r2=0.979).The results indicated that the developed method was a simple,sensitive,accurate and cost-effective approach for the screening of OTC in food samples. |
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