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Detection of Riboflavin Based on Sodium Bisulfite-Hydrogen Peroxide Ultra-weak Chemiluminescence System |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:chemiluminescence sodium bisulfite hydrogen peroxide riboflavin |
Author | Institution |
LIN Zhen,ZHU Lian-jie,WANG Fei-fei,WU Na-mei,LIN Xin-hua |
福建医科大学药学院药物分析系 |
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Abstract: |
The reaction between NaHSO3 and H2O2 produced ultra-weak chemiluminescence(CL).Riboflavin could greatly enhance the CL from NaHSO3-H2O2 system.The CL intensity was linearly increased with concentration of riboflavin in the range of 0.02-2.0 μg/mL.Based on the phenomenon,a high sensitive chemiluminescent method was developed for the determination of riboflavin in tablet samples.The detection limit was 0.007 μg/mL and the relative standard deviation was not more than 1.6%.Furthermore,fluorescent and chemiluminescent spectrum was utilized to illustrate the chemiluminescent mechanism,which would extend the application of NaHSO3-H2O2 system. |
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