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Preparation of Ag@SiO2/ZnO@SiO2 and Its Application in Detection of Biological Molecules in Aqueous Solution |
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DOI: |
KeyWord:zinc oxide nano silver fluorescence stability analysis of test hemoglobin L-cysteine |
Author | Institution |
HUANG Wen-yi,WEI Hui-dan,SHI Zhi-peng,BAI Da-wei,MA Lan-yu,LI Li-jun* |
广西科技大学生物与化学工程学院,广西糖资源绿色加工重点实验室,广西高校糖资源
加工重点实验室 |
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Abstract: |
A simple,rapid and mild condition method was developed for the preparation of ZnO@SiO2 core-shell structures quantum dots and Ag@SiO2/ZnO@SiO2 nanocomposite.Particle sizes,UV-vis absorption and emission spectra were characterized by transmission electron microscopy(TEM),ultraviolet spectrometry(UV),fluorescence spectroscopy(FL),nanometer particle size analyzer and ZETA potentials techniques.The results showed that the particles sizes of ZnO@SiO2 core-shell structures quantum dots were 3-8 nm and could emit yellow green fluorescence under the excitation of UV-vis light.Their maximum absorption wavelength was 330 nm,and the maximum fluorescence emission wavelengths were located at 510 nm.Ag@SiO2/ZnO@SiO2 could enhance the fluorescent of ZnO@SiO2 core-shell quantum dot,but it didn-t - change the emission peak position of ZnO@SiO2 core-shell quantum dot.Otherwise,Ag@SiO2/ZnO@SiO2 greatly enhanced the fluorescent stability of ZnO@SiO2 core-shell quantum dots at room temperature.Based on different level quenching of hemoglobin and L-cysteine for quantum dots,a simple,rapid and sensitive detection method was established.Under the optimized conditions,the characteristic fluorescence intensity of Ag@SiO2/ZnO@SiO2 at 510 nm was linearly proportional to the concentration of L-cysteine and hemoglobin in ranges of 0.068-10.10 mg/L and 4.00×102-4.00×103 mg/L,respectively.The detection limits were 6.92×10-3 mg/L and 2.04 mg/L,respectively,and the correlation coefficients were 0.993 8 and 0.995 1,respectively.The relative standard deviations for 11 replicate detections of 3.37 mg/L L-cysteine and 2.00×103 mg/L hemoglobin were 1.3% and 2.5%,respectively. |
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