Separation and Characterization of Phenolic Compounds in Chuju by Two-dimensional Hydrophilic Interaction/Reversed Phase Liquid Chromatography Coupled to Diode Array Detection

KeyWord:Chuju  phenolic compounds  comprehensive two-dimensional liquid chromatography  hydrophilic interaction/reversed phase liquid chromatography£¨HILIC /RPLC£©

Abstract:

      An innovative analytical approach based on the use of comprehensive two-dimensional hydrophilic interaction/reversed phase liquid chromatography£¨HILIC/RPLC£© was applied to obtain the profiling of phenolic compounds in Chuju.The method involved the use of a microbore hydrophilic interaction column containing a diol stationary phase£¨Lichrospher diol-5£© in the first dimension combined with a reversed phase column£¨Cortecs C18+£© in the second dimension£¬and applied two sample loops as interface between the first and the second dimension.Two different gradient elutions were employed in the two dimensions£¬respectively.Detecton wavelength was set at 330 nm,and sampling rate was 15 Hz in the diode array detection.The theoretical peak capacity was up to 1 117 while the surface coverage proposed by Gilar£¨SCG£© was 0.56£¬indicating both good resolution and good orthogonality of this 2D-LC system.23 peaks in the 2D-LC separation space were found£¬and 13£¨apigenin£¬luteolin£¬acacetin£¬acacetin-7-O-glucoside£¬chlorogenic acid£¬isorhamnetin-3-O-glucoside£¬luteolin-7-O-glucoside£¬acacetin-7-O-rutinoside£¬acacetin-7-O-rutinoside£¬quercitrin£¬isochlorogenic acid A£¬isochlorogenic acid B£¬apioside£©of them were allowed tentative identification according to comparing with retention times and maximum absorption wavelengths between the sample and standards.In which 4 phenolic compounds£¨isorhamnetin-3-O-glucoside£¬acacetin-7-O-rutinoside£¬eriodictyol-7-O-glucoside£¬apioside£©were firstly found in Chuju and isorhamnetin-3-O-glucoside was firstly found in Dendranthema morifolium.