Investigation on Interaction of Doxorubicin Hydrochloride with Human Telomere DNA by Electrochemical Methods

KeyWord:electrochemistry  doxorubicin hydrochloride  human telomere DNA  interaction  UV-Vis spectrometry

Abstract:

      Electrochemical behavior of the anticancer doxorubicin hydrochloride£¨DOX£©and the interaction of DOX with human telomere DNA were investigated by cyclic voltammetry in aqueous medium using pH 6.0 PBS buffer.The results showed that a pair of reversible oxidation-reduction peaks were observed at -0.585 V and -0.638 V in pH 6.0 PBS buffer.There existed excellent linearities between oxidation peak current and concentration of DOX in the ranges of 0.03-0.8 ¦Ìmol/L and 0.8-10 ¦Ìmol/L by linear sweep voltammetric method.Detection limit£¨S/N=3£© was 0.01 ¦Ìmol/L.The relative standard deviations of the oxidation peak current obtained from 11 determinations of the same solutions containing 0.5 ¦Ìmol/L DOX were 3.5%.The oxidation peak current of DOX gradually decreased with the adding of various concentrations of human telomere DNA£¬due to interaction of DOX with human telomere DNA.The binding ratio was calculated to be 1¡Ã1 and the binding equilibrium constant was 1.11¡Á103 L/mol.