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Study on Interaction of GR-TiO2 with Lysozyme and Its Influence on Enzyme Activity by Spectroscopy |
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DOI: |
KeyWord:graphene-TiO2 nano-TiO2 lysozyme spectroscopy enzyme activity |
Author | Institution |
FU Zheng-qing,TONG Yue-ju,WANG Ling,LIANG Hong-li,LI Sha,ZHANG Ai-ping* |
山西医科大学药学院 |
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Abstract: |
Under the simulative human physiological conditions,using nano-TiO2 as the control,the interaction of GR-TiO2 with lysozyme(LYSO) was investigated by ultraviolet spectrometry,fluorescence spectroscopy,synchronous fluorescence spectroscopy,three dimensional fluorescence spectroscopy and circular dichroism.The enzyme activity was determined in the presence of GR-TiO2.The results of fluorescence spectroscopy revealed that the endogenous fluorescence of LYSO was quenched by GR-TiO2 and its mechanism was static quenching.The major driving forces were hydrogen bonds and van der Waals′ forces.These results of GR-TiO2 were consistent with that of nano-TiO2.The results of synchronous fluorescence and circular dichroism spectra further demonstrated that the conformation of LYSO influenced by GR-TiO2 was more than that by nano TiO2.The result of enzyme activity indicated that the enzyme activity of LYSO was inhibited more strongly by GR-TiO2. |
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