|
Magnetic Affinity Immunoassay Based on Gold-magnetic Particle and HRP-conjugated Phage Antibody |
|
View Full Text Download reader |
DOI: |
KeyWord:gold-magnetic particle functionalized probe HRP-conjugated phage antibody abrin detection |
Author | Institution |
MU Xi-hui,TONG Zhao-yang*,HUANG Qi-bin,LIU Bing,LIU Zhi-wei,HAO Lan-qun,ZHANG Jin-ping |
国民核生化灾害防护国家重点实验室 |
|
Hits: 2169 |
Download times: 985 |
Abstract: |
A new magnetic affinity immunoassay(MAIA) method based on gold-magnetic particle and HRP-conjugated phage antibody was developed.Gold-magnetic particle were coated with staphylococcus protein A(SPA) and coupled with polyclonal antibody(pcAb) to construct functionalized capturing probes,and HRP-conjugated phage antibody was then used as specific detecting probe to design MAIA for trace detection of abrin.The calibration curve was linear(r=0.991 0) in the range of 0.008-250 μg/L,with a limit of detection(LOD) of 0.008 μg/L,and a limit of quantitation(LOQ) of 0.008 μg/L.Integrating the unique advantages of functionalized SPA gold-magnetic particle probe and HRP-conjugated phage antibody with its high sensitivity,specificity and anti-interference,the method is proven to be well suited for the analysis of trace abrin in various environmental samples. |
Close |
|
|
|