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| Determination of Cefepime by Luminol-K3［Fe(CN)6］ Flow Injection Chemiluminescence System |
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| DOI： |
| KeyWord:chemiluminescence enhencement flow injection cefepime luminol potassium ferricyanide |
| Author | Institution |
| YU Hong-song,ZHAI Qian,LI Xiao-lu,GUO Jing,YI Gang* |
重庆医科大学检验医学院临床检验诊断学教育部重点实验室 |
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| Abstract: |
| In this paper,cefepime was found to enhance the chemiluminescence(CL)of the luminol-potassium ferricyanide reaction system in alkaline condition.According to this fact,a flow injection chemiluminescence enhencement method for the determination of cefepime was developed.Under the optimum conditions,the linear range for cefepime was 3.0×10-6-4.0×10-5 g/mL,and the limit of detection was 2.8×10-6 g/mL.The relative standard deviation(RSD,n=11) was 1.5%for the determination of 2.0×10-5 g/mL cefepime.The method was applied in the determination of cefepime injection with satisfactory results. |
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