Determination of Resveratrol in Peanut Root Using Molecularly Imprinted Polymers Multiplug Filtration Clean up Column Coupled with Fluorescence Spectrometry

KeyWord:resveratrol  molecular imprinted polymers  multiplug filtration clean-up column  molecular fluorescence spectrometry  peanut root

Abstract:

      New molecularly imprinted polymers£¨MIPs£© were prepared by using attapulgite£¨ATP£© as the support,¦Â-CD and methacrylic acid£¨MAA£© as functional monomer,resveratrol as template molecule through surface molecularly imprinted polymerization technology.The MIPs were used as sorbent filled into the multiplug filtration clean-up£¨m-PFC£© column for resveratrol enrichment and purification.A new method was developed for the determination of resveratrol by using multiplug filtration clean-up column with molecularly imprinted polymers extraction£¨MIPs-m-PFCE£© combined with fluorescence spectrometry.The results showed that the present method has a high selectivity for RES under the optimized experimental conditions,the adsorption capacity of MIPs was 12.78 mg/g.The calibration curve was linear in the range of 0.1-100 ¦Ìg/mL with a correlation coefficient£¨r£© of 0.999 7.The limit of detection was 0.048 ¦Ìg/L.The proposed method was successfully applied in the determination of RES in peanut root,with average recoveries of 86.2%-102.1% and relative standard deviations£¨RSDs£© of 3.5%-5.8% at three spiked levels.The developed method is rapid and selective,and is adaptable to the analysis of trace RES in real samples.