Determination of Lauroyl Glutamate Content in Coconut Oleoyl Glutamate by High Performance Liquid Chromatography

KeyWord:high performance liquid chromatography£¨HPLC£©  cocoyl glutamate acid  lauroyl glutamate

Abstract:

      A high performance liquid chromatographic£¨HPLC£© method was developed to detect the content of lauroyl glutamate in coconut oleoyl glutamate surfactant and its sodium salts.The sample was dissolved with methanol in vortex mixer£¬and separated on an Agilent TC-C18£¨250 mm¡Á4.6 mm¡Á5 ¦Ìm£© column using acetonitrile-0.002 mmol/L H2SO4 as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm;The injection volume was 10 ¦ÌL.Under the optimal conditions,the calibration curve was linear in the range of 0.050-2.0 mg/mL with a detection limit£¨S/N=3£© of 0.015 mg/mL.The recoveries were between 92.0% and 101%.With the advantages of accurancy,rapidness and good reproducibility,this method is suitable for the determination of cocoyl glutamate acid in different quality of fatty acids,and provides an effective reference for the quality control of this surfactants.