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Study on Interaction between Antitumor Drug and DNA by Three-dimensionally Ordered Gold-nanoparticle-doped Titanium Dioxide Modified Electrode |
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DOI: |
KeyWord:Three-dimensionally ordered macroporous(3DOM) gold-nanoparticle-doped titaniumdioxide(GTD) DNA modified electrode quercetin (Qu) intercalation sequence selectivity |
Author | Institution |
PENG Xin-sheng,ZHU Li-li,LIU Yan-rui,DU Jiang-yan* |
1.南京师范大学化学与材料科学学院;2.江苏省生物功能材料重点实验室;3.江苏省新型动力电池重点实验室 |
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Abstract: |
A novel DNA biosensor was fabricated with three-dimensionally ordered macroporous gold-nanoparticle-doped titanium dioxide(3DOM GTD) film on the indium-tin oxide(ITO) electrode surface by the colloidal crystal template technique,and calf thymus DNA(ctDNA)was immobilized on the 3DOM GTD/ITO successfully.The morphology of the modified electrode was characterized by transmission electron microscopy(TEM) and scanning electron microscopy(SEM).The immobilization of ctDNA on the surface of 3DOM GTD/ITO modified electrode was investigated by electrochemical impedance spectroscopy(EIS).The EIS experimental results showed that ctDNA was successfully immobilized on the 3DOM GTD/ITO modified electrode.The electrochemical properties of the antitumor drug quercetin (Qu) and the interaction between Qu and ctDNA were studied on 3DOM GTD/ITO modified electrode by cyclic voltammograms (CV) and differential pulse voltammograms(DPV) method.The result showed that Qu had a pair of quasi-reversible redox peak on 3DOM GTD/ITO modified electrode,and the transfer process of 2 electrons and 2 protons was an involved in the electrode reaction.There was a strong interaction between Qu and ctDNA immobilized on the modified electrode,and the binding constant K was 3.61×106L/mol.The binding mode between Qu and ctDNA was an intercalation confirmed by CV,UV-Vis absorption spectra (UV-Vis),fluorescence spectra(FL) and circular dichroism spectra(CD) experiments. The sequence selectivity of binding between Qu and ctDNA was investigated,and the binding constants of Qu and poly(dG-dC) and poly(dA-dT) were calculated, respectively, according to the results of CV experiments.The binding constant ratio K(dG-dC)/K(dA-dT) was obtained to be 3.5,indicating that Qu bound to the GC region preferentially when Qu interacted with ctDNA. |
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