Rapid Analysis of Perfluorinated Compounds in Coastal and Estuarine Seawater by SPE Enrichment with Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:perfluorinated compounds  coastal and estuarine seawater  ultra high performance liquid chromatography tandem mass spectrometry£¨UHPLC-MS/MS£©  solid phase extraction

Abstract:

      A rapid method was developed for the quantitative analysis of perfluorinated compounds£¨PFCs) including perfluorooctane sulfonic acid£¨PFOS£©,perfluorooctanoic acid£¨PFOA£©,perfluoroundecanoic acid£¨PFUnA£©,perfluorododecanoic acid£¨PFDoA£©,perfluorotridecanoic acid£¨PFTrDA£©,perfluorotetradecanoic acid£¨PFTA£© in coastal and estuarine seawater by solid phase extraction£¨SPE£© enrichment with ultra high performance liquid chromatography tandem electrospray ionization mass spectrometry£¨UHPLC-MS/MS£©.Seawater sample was enriched and purified through a 500 mg C18 SPE cartridge at 5.0 mL/min,eluted with 15 mL mixed solvent of methanol and ethyl acetate£¨4¡Ã1£©, and finally concentrated into 1.0 mL with an automated de-solvent device.Target analytes were separated on a Kinetex XB-C18 column using methanol£¨containing 5.0 mmol/L NH4COOH-water£¨containing 5.0 mmol/L NH4COOH£© as mobile phase.The negative electrospray ionization£¨ESI-£© and multiple reaction monitoring mode£¨MRM£© were utilized.An internal standard calibration method was chosen for the quantitative analysis.The calibration curves of PFCs were linear in the range of 0.5-50.0 ¦Ìg/L with correlation coefficients more than 0.999 0.The limits of quantitation£¨LOQs,S/N=10£© were between 0.5 ng/L and 1.5 ng/L.The recoveries of 6 PFCs at three spiked levels£¨2.0,5.0,10.0 ng/L£© were in the range of 80.1%-117.4%,and relative standard deviations£¨RSDs£© were 8.2%-12.1% at spiked level of 2.0 ng/L.