Highly Efficient Enrichment of Bacteria Aerosol Based on a Single Spiral Microfluidic Chip

KeyWord:single spiral microfluidic chip  Vibrio parahaemolyticus  enrichment  herringbone structure

Abstract:

      Highly efficient enrichment is the key for rapid analysis and detection of microbiological aerosol.Herein,a poly(dimethylsiloxane)(PDMS) microfluidic chip which has a single spiral channel was developed,and the chip was used for Vibrio parahaemolyticus enrichment.The characteristic of this chip is that it has a single spiral channel which contains a herringbone structure with uniform distribution.During different enrichment times,the number of captured bacteria by microfluidic chip is far bigger than that by the traditional sediment method.Moreover,the chip shows more enrichment advantages when capturing microbiological aerosol in low concentration.This excellent enrichment ability,on one hand,benefits from the unique spiral channel design which can put greater centrifugal force to the microbiological aerosol.On the other hand,the herringbone structures design endows the chip larger surface specific area increasing the contact probability between microbiological aerosol and chip.In addition,a similar spiral microfluidic chip without herringbone structures was also designed.The results validated the contribution of herringbone structures to the enrichment efficiency. With the advantages of delicate design and fabrication,portability and high enrichment capacity,this microfluidic chip shows a great application potential in field like aquatic products processing industry.