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Analysis of Sulfonamide,Quinolones,Steroid Hormones and Tetracyclines Residues in Milk by Ultra Performance Liquid Chromatography-Mass Spectrometry |
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DOI: |
KeyWord:ultra performance liquid chromatography-mass spectrometry(UPLC-MS) milk veterinary drugs residues sulfonamides quinolones steroid hormones tetracycline |
Author | Institution |
LI Ning,ZHANG Yu-long,LIN Tao,WANG Ji-liang,LIU Hong-cheng |
1.昆明医科大学药学院;2.云南省农业科学院质量标准与检测技术研究所 |
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Abstract: |
A comprehensive method was developed and optimized for the simultaneous analysis of sulfonamides,quinolones,steroid hormones and tetracycline by ultra performance liquid chromatography-mass spectrometry(UPLC-MS).The veterinary drugs in milk samples were extracted with 5% acetic acid-acetonitrile solution,and then the mixture was centrifuged.The supernatant was purified with an HLB solid phase extraction column,and analysed by UPLC-MS and quantified by the external standard method.Average recoveries of 25 veterinary drugs at three spiked levels ranged from 61.6% to 119.2% with relative standard deviations(RSDs) of 2.5%-13.4%.The method limits of detection(LOD) for target compounds were in the range of 0.5-2.0 μg/kg. |
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