Determination of Nine Volatile Nitrosamines in Local Sausage on Sale by Gas Chromatography Tandem Triple Quadrupole Mass Spectrometry

KeyWord:volatile nitrosamines  gas chromatography tandem triple quadrupole mass spectrometry  local sausage

Abstract:

      A selective gas chromatography tandem triple quadrupole mass spectrometry was used to detect nine volatile nitrosamines,including N-nitrosodimethylamine(NDMA),N-nitrosodiethylamine(NDEA),N-nitrosomethylethylamine(NMEA),N-nitrosodipropylamine(NDPA),N-nitrosopyrrolidine(NPYR),N-nitrosopiperidine(NPIP),N-nitrosodibutylamine(NDBA),N-nitrosomorpholine(NMOR),N-nitrosodiphenylamine(NDPhA) in local sausage on sale by using QuEChERS sample pretreatment.The calibration curves of nine volatile nitrosamines were linear in the range of 0.25-200 ¦Ìg/L,with detection limits of 0.01-0.10 ¦Ìg/kg.The recoveries were in the range of 90.7%-116.0% at three spiked concentration levels in sample,with relative standard deviations(RSDs) of 1.8%-14.0%.The method was applied in the detection of local sausage on sale,and seven volatile nitrosamines were all detected.The detection rates of NDMA£¬NMOR£¬NPYR£¬NDPA were higher than that of the others.The contents of total volatile nitrosamines were in the range of 1.85-13.44 ¦Ìg/kg.The developed method was simple,rapid,sensitive and accurate.