Simultaneous Determination of 14 Forbidden Colorants in Cosmetics by HPLC-MS/MS

KeyWord:cosmetics  forbidden colorants  HPLC-MS/MS

Abstract:

      An HPLC-MS/MS methord was developed for the simultaneous determination of 14 forbidden colorants,including metanil yellow,acid violet 49,solvent red 49,solvent blue 35,tetramethylpararosaniline chloride£¨TMPC£©,pentamethyltriphenylmethane£¨PMTPE£©,hexamethylenepara rosaniline chloride£¨HMPC£©,pigment orange 5,pigment red 53,sudan I,sudan¢ò,Sudan ¢ô,rhodamine B,disperse yellow 3,in cosmetics.Cosmetic samples were extracted and dispersed with tetrahydrofuran,and some matrixes were precipitated out after addition of ammonium acetate solution and methanol.After centrifuged and filtered£¬the extract was separated on an Agilent poroshell 120 EC-C18 column£¨2.7 ¦Ìm£¬3.0 mm¡Á50 mm£©by gradient elution with 10 mmol/L ammonium acetate-acetonitrile as mobile phase.The quantitative analysis was performed by the standard curve method of peak area.The linear ranges of 14 analytes were in the range of 0.01-1.0 ¦Ìg/mL,with correlation coefficients more than 0.999.The limits of quantitation£¨LOQs£©were in the range of 0.05-0.5 ¦Ìg/g.The average recoveries at three spiked levels ranged from 85.4% to 106.3% with RSDs less than 10%.This method is accurate,simple,sensitive and reliable,and could be used for the analysis of 14 forbidden colorants in cosmetics.