Determination of 10 Kinds of Fungicides in Leather by Ultrasonic Extraction Combined with Ultrahigh Performance Liquid Chromatography

KeyWord:leather  fungicide  ultrasonic extraction  ultrahigh performance liquid chromatography£¨UPLC£©

Abstract:

      A new method was established to determine 10 kinds of fungicides£¨including sulfanilamide£¬benzimidazole£¬BIT£¬MBT£¬IPBC£¬PCMC£¬salicylanilide£¬OPP£¬TCMTB£¬OIT£© in leather samples by ultrasonic extraction combined with ultrahigh performance liquid chromatography£¨UPLC£©.The leather samples were ultrasonically extracted with methanol£¬and separated with T3 column£¬employing methanol and 0.1%£¨by volume£© formic water as mobile phase by gradient elution£®All of the samples were detected with diode-array detector.The 10 kinds of analytes presented favorable linearity in a certaim concentration range£¬with correlation coefficients£¨r2£© not less than 0.999 3.The method quantitation limit£¨LOQ£¬S/N=10£© of IPBC was 190.0 mg/kg£¬and the LOQs of other compounds were in the range of 4.0-8.0 mg/kg.The average recoveries£¨n=6£© ranged from 81.3% to 104.9%£¬and the relative standard deviations £¨RSD£¬n=6£© were in the range of 4.7%-11.2%.With the advantages of simple pre-treatment procedure and satisfactory separate effect£¬the developed method was suitable for the simultaneous determination of 10 kinds of fungicides in leather samples.