Determination of 15N Labeled Arginine Fermentation Broth and Isotope Abundance by GC-MS

KeyWord:gas chromatography-mass spectrometry£¨GC-MS£©  derivatization  amino acids  15N labeled amino acids  isotope abundance

Abstract:

      A method for the determination of arginine£¬lysine£¬serine£¬valine and other eleven amino acids was developed by MSTFA derivatization combined with gas chromatography-mass spectrometry£¨GC-MS£©£¬and fifteen amino acids reached the baseline separation.The arginine fermentation broth was purified by cationic exchange mixed-solid phase extraction£¨SPE£©£¬ and then detected successfully by the developed method.Finally the 15N isotope abundance was calculated by characteristic fragment.