Determination of Benzoic Acid£¬Sorbic Acid£¬Acesulfame K and Saccharin Sodium in Honey by Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry

KeyWord:high resolution mass spectrometry£¨HRMS£©  ultra high performance liquid chromatography£¨UPLC£©  preservative  sweetener

Abstract:

      A method based on ultra high performance liquid chromatography-high resolution mass spectrometry was developed to separate benzoic acid£¬sorbic acid£¬acesulfame-K and saccharin sodium in honey.The targeted compounds in honey samples were extracted with methanol-water mixtures£¨10¡Ã90£©,and separated on a Diamonsil Plus C18 column with 0.5 mmol/L ammonium acetate-methanol as mobile phase by gradient elution.The qualitative and quantitative analyses were operated under t-MS2 by high resolution mass spectrometry with the external standard method.The results indicated that four analytes had good linearities in the concentration range of 0.02-2.0 mg/L with correlation coefficients of 0.998 7-0.999 7.The quantilition limits for benzoic acid£¬sorbic acid,acesulfame-K and saccharin sodium were 0.5£¬0.5£¬0.3,0.3 mg/kg£¬respectively.The recoveries of four compounds at three spiked levels were in the range of 89.0%-104.0%£¬with relative standard deviations£¨RSDs£© of 2.0%-7.9%.The method was simple and rapid£¬and was suitable for the determination of benzoic acid£¬sorbic acid and acesulfame-K£¬saccharin sodium in honey.