Study on Carbon Dots-Fluorescein Fluorescence Resonance Energy Transfer System and Its Application in Aspirin Analysis

KeyWord:carbon dots  fluorescein  fluorescence resonance energy transfer£¨FRET£©  aspirin

Abstract:

      A fluorescence resonance energy transfer£¨FRET£©system between fluorescence carbon dots£¨CDs,donor£©and fluorescein£¨FAM,acceptor£©was constructed,and a novel method for the sensitive and selective determination of aspirin£¨ASP£©was accordingly proposed.It was found that in pH 7.0 Tris-HCl buffer£¬effective energy transfer from CDs to FAM occurred,which resulted in a great enhancement of the fluorescence intensity of FAM.Upon the addition of ASP,fluorescence quenching of FAM occurred,and the change of FAM fluorescence intensity was in a good linearity£¨r=0.999 6£©with ASP in the concentration range of 1.0-150.0 ¦Ìg/mL.A novel fluorescence quenching platform for the determination of ASP was constructed.Under the optimum conditions,the detection limit of ASP for this method was 0.33 ¦Ìg/mL£¨3¦Ä/k,n=11£©,and the spiked recoveries ranged from 97.1%to 105.3%with RSD£¨n=6£©not more than 4.6%.The result showed that common relevant substance,cations and anions did not interfere with the detection of ASP.