Determination of Polycyclic Aromatic Hydrocarbons£¨PAHs£© in Plant Leaf Samples by Silica Gel Column and Gel Column Purification/Gas Chromatography-Mass Spectrometry

KeyWord:soxhlet extraction  silica gel column  gel permeation chromatography£¨GPC£©  polycyclic aromatic hydrocarbons£¨PAHs£©  gas chromatography-mass spectrometry£¨GC-MS£©  plant

Abstract:

      A method was developed for the analysis of polycyclic aromatic hydrocarbons£¨PAHs£© in plant leaves by gas chromatography-mass spectrometry£¨GC-MS£© based on silica gel column and gel permeation chromatography£¨GPC£© purification.The target analytes in leaf samples were extracted with dichloromethane-hexane£¨1¡Ã1£¬by volume£© solution£¬purified with silica gel column and GPC column£¬analyzed by GC-MS£¬and then quantified by the internal standard method.The average recoveries of 15 PAHs were in the range of 82.2%-130% with relative standard deviations£¨RSD£© not more than 11%.The detection limit£¨LOD£© of this method varied from 0.025 1 ng?g-1 to 5.80 ng?g-1.This method could effectively remove the pigment and oil in the plant leaves£¬suitable for the trace analysis of PAHs in leaf samples£¬and providing a technical support for the determination of content of PAHs in the plant tissues.