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| Rapid Determination of Polyether Antibiotics Residues in Animal-originated Foodstuffs Using TurboFlow On-line Clean-up Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:TurboFlow on-line cleanup liquid chromatography-tandem mass spectrometry polyether antibiotics animal-originated foodstuffs |
| Author | Institution |
| CAO Ya-qing， SONG Ge， WANG Man-man， WANG Xue-sheng， AI Lian-feng |
1.华北理工大学公共卫生学院;2.河北出入境检验检疫局 |
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| Abstract: |
| A new method using TurboFlow online cleanup combined with tandem mass spectrometry was developed for the rapid and simultaneous determination of six polyether antibiotics residues,e.g.lasalocid,salinomicin,monensin,narasin,maduramycin and nigericin in animal-originated foods of animal origin.The milk，chicken and egg samples were extracted with acetonitrile，and the extracts were purified on a Cyclone-p column.After being eluted from the clean-up column，the analytes were separated on a Thermo Scientific Hypersil-Gold C8(150 mm×2.1 mm，5 μm) column and detected by electrospray mass spectrometry in positive ionization mode with multiple reaction monitoring.Under the optimized conditions，the linear ranges of six polyether antibiotics were in the range of 1-100 μg/L with correlation coefficients higher than 0.999.The limits of quantitation(LOQ) for all samples were 1 μg/kg.The recoveries from milk，chicken and egg samples were in the range of 71.9%-109.0% with relative standard deviations of 1.5%-14.9%. |
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