Lung Metabolome Research of Rats Exposed to Tobacco Smoke with Herbal Additives by Liquid Chromatography-Mass Spectrometry

KeyWord:liquid chromatography-mass spectrometry(LC-MS)  metabonomics  tobacco with herbal additives  lung  biomarker

Abstract:

      The effect of tobacco with herbal additives on the global metabolome of rat lung was studied using liquid chromatography-mass spectrometry(LC-MS).Lung samples from control rats£¬normal tobacco smoked rats and a certain commercial tobacco(a specific tobacco with herbal additives)smoked rats were analyzed£¬and the groups were exposed to smoke for one week£¬half month and one month£¬respectively.The data were processed by the method of partial least squares discriminant analysis(PLS-DA).The potential biomarkers were screened out according to VIP(Variable Importance in the Projection) and identified by database and standards.The PLS-DA results of different tobacco exposed periods showed that the metabolism of both the normal tobacco exposed group and the certain commercial tobacco exposed group were affected£¬mainly including phospholipid metabolism and fatty acid metabolism. In particular£¬two lung samples from rats exposed to normal tobacco were abnormal significantly as a result of suffering from a serious oxidative damage. Overall£¬especially prolonged exposure to smoke£¬the smoke damage of the certain commercial tobacco smoked rats was lower than that of the normal tobacco smoked rats£¬indicating that adding some herbal additives to tobacco could contribute at least partly to reduce the harm caused by smoking.