Determination of 67 Pesticide Residues in Vegetables and Fruits by Gas Chromatography-Triple Quadrupole Mass Spectrometry with Dispersive Solid Phase Extraction

KeyWord:gas chromatography-triple quadrupole mass spectrometry£¨GC-MS/MS£©  dispersive solid phase extraction£¨QuEChERS£©  vegetables  fruits  pesticide residues

Abstract:

      An analytical method for the simultaneous determination of 67 pesticides in vegetables and fruits by gas chromatography-triple quadrupole mass spectrometry£¨GC-MS/MS£© with dispersive solid phase extraction£¨QuEChERS£©.The samples were extracted with acetonitrile£¨containing 1% acetic acid£©£¬and then cleaned up with dispersive solid phase extraction.The extracts were determined by GC-MS/MS in multi-reaction monitoring(MRM) mode with the external standard method. The calibration curves of 67 pecitides were linear in the range of 0.005-0.24¦Ìg/mL with correlation coefficients£¨r2£© higher than 0.99.The average recoveries at spiked concentration levels of 0.02£¬0.06£¬0.12mg/kg ranged from 60.3% to 119.8% with relative standard deviations£¨RSDs£© of 0.5%-14.7%.The limits of detection£¨LOD£© for the method were in the range of 0.0001-0.003mg/kg.The limits of quantitations£¨LOQs£© were 0.0002-0.009mg/kg.The method was applied in the detection of pesticide residues of vegetables and fruits£¬and the result was more satisfactory comparing with that of GC£¬GCP/GC-MS£¬LC-MS/MS methods. The method showed the advantages of simplicity, quickness, high sensitivity£¬good selectivity£¬good reproducibility and stability£¬and was suitable for the analysis of pesticide residues in daily vegetables and fruits.