Direct Analysis of Aflatoxin B1 in Rice by Microwave Plasma Torch Mass Spectrometry

KeyWord:microwave plasma torch£¨MPT£©  mass spectrometry  aflatoxin B1£¨AFB1£©  rice

Abstract:

      A novel and simple mass spectrometric method based on microwave plasma torch£¨MPT£© was developed for the rapid detection of aflatoxin B1£¨AFB1£© in rice without sample pretreatment.The MPT mass spectra of AFB1 were obtained,and the quantitative analysis mainly was based on the characteristic fragments of AFB1£¨m/z 285£©.The results showed that there was a good linear relationship in the concentration range of 0.5-20 ¦Ìg/L with correlation coefficient£¨r2£© of 0.999 4£¬and the evaluated limit of detection£¨LOD£© was 0.12 ¦Ìg/kg.The direct detection of AFB1 in some kinds of rice were conducted£¬with recoveries of 95.8%-107.2% and relative standard deviations£¨RSD£¬n=9£© of 2.2%-10.1%ª±With the help of principle component analysis£¨PCA£©£¬the moldy rice and normal rice could be rapidly distinguishedª±With the advantages of rapidness and high sensitivity£¬the MPT-MS is a powerful tool for the high-throughput detection of moldy rice,and also shows a great potential in the monitoring of cereal and oil£¬animal£¬plant food£¬etc.