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| Determination of Ellagic Acid in Strawberry by UPLC-MS/MS |
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| DOI： |
| KeyWord:ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) free ellagic acid total ellagic acid strawberry |
| Author | Institution |
| YANG Yuan，SHI Lei，YANG Jun-jun，ZHANG Ying-ying，ZHANG Kai-chun* |
1.北京市农林科学院林业果树研究所;2.农业部华北地区园艺作物生物学与种质创制重点实验室;3.北京市落叶果树工程技术研究中心 |
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| Abstract: |
| A method was developed for the determination of ellagic acid(free ellagic acid and total ellagic acid) in strawberry by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).In this work，the free ellagic acid was extracted from strawberry with 0.1%trifloroacetric acid-methane，purified by C18 dispersive solid phase extraction，and determined by UPLC-MS/MS.Total ellagic acid was hydrolyzed to free ellagic acid with 2 mol/L trifloroacetric acid aqueous，then purified with C18 dispersive solid-phase extraction column，and determined by UPLC-MS/MS.Sample solution was separated on a C18 column by gradient elution using methanol-0.5%formic acid aqueous as mobile phase.The linear range of 10-500 ng/mL for ellagic acid was obtained，with correlation coefficient of 0.998 1.The limit of quantitation was 0.5 mg/kg for free ellagic acid and 5.0 mg/kg for total ellagic acid.The recoveries at low，medium and high spiked levels ranged from 867%to 1136%with relative standard deviations(RSD) not more than 10%.The results indicated that this approach was simple，sensitive and accurate，and was applicable for the determination of content of ellagic acid in strawberry. |
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