Rapid Determination of Coumarin in Chinese Traditional Patent Medicine and Raw Materials by UPC2 and Solvent Induced Extraction

KeyWord:coumarin  solvent induced extraction  ultra performance convergence chromatography£¨UPC2£©  ultra performance liquid chromatography£¨UPLC£©  detection

Abstract:

      A method of solvent induced phase extraction and ultra performance convergence chromatography£¨UPC2£© was established for the determination of coumarin in Chinese traditional patent medicine and raw materials.Samples were crushed£¨60 mesh£©£¬and extracted with acetonitrile-water£¨7¡Ã3,by volume£© using dichloromethane as induced separation solvent.The targeted compound was detected by UPC2 method.The limit of detection£¨LOD,S/N=3£© for coumarin was 0.1 mg/L.The linear range of coumarin was in the range of 0.3-10.0 mg/L.The spiked recoveries ranged from 89.8% to 102.3% and the relative standard deviations were between 0.31% and 1.0%.Compared with UPLC method£¬the method showed the advantages of high selectivity and low detection cost£¬and its analysis time is only 1/4 of the UPLC.This study provided a new pretreatment and detection method for the high throughput detection of the coumarin.