Identification of Starch Syrup in Honey Adulteration Based on Oligosaccharide Profiling Test by High Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbitrap High Resolution Mass Spectrometry

KeyWord:honey adulteration  oligosaccharide profiling test  high performance liquid chromatography-quadrurpole/electrostatic field Orbitrap high resolution mass spectrometry

Abstract:

      A method of oligosaccharide profiling test was developed based on high performance liquid chromatography-quadrurpole/ electrostatic field orbitrap high resolution mass spectrometry(HPLC-Q/Orbitrap MS).Through qualitative and quantitative analysis of maltotriose£¬maltotetraose£¬maltopentaose£¬maltohexaose and maltoheptaose in pure natural honey and starch syrup£¬trace of malt oligosaccharides that was not totally enzymatic hydrolyzed were found in starch syrup.Furthermore£¬the interference of maltoheptaose in all test samples was the smallest and had no impact on the quantitative analysis results.While£¬the qualitative or quantitative analysis of other oligosaccharides were interfered more or less.In the analysis of real samples£¬the starch syrup in honey adulteration could be judged by the retention time and relative abundance ratio of maltoheptaose£¬therefore the content of maltoheptaose in sample solution could be quantitatively determined through standard curve method.By adding standard test£¬the average recoveries of maltoheptaose at three spiked levels of 20£¬50 and 100 mg/kg were in the range of 75%-82% with relative standard deviations(RSD£¬n=5) of 3.1%-6.7%.The detection limit of this method was 0.1 ¦Ìg/mL.The whole analysis time could be limited in 10 min.The method had the advantages of simple processing procedure£¬high accuracy£¬reliability£¬sensitivity£¬therefore it could be applied in the rapid detection of starch syrup in honey.