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| Preparation and Characterization of Gigaporous Polymer Affinity Chromatographic Supports |
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| DOI： |
| KeyWord:affinity chromatography coupling method Protein A ligand gigaporous chromatographic support purification of antibody |
| Author | Institution |
| ZHANG Rong-yue*，CHEN Xuan-you，ZHANG Yi，JIANG Cheng-wei，HUANG Yi-kang，LI Qiang，DING Hong，PAN Yi-ting，LIU Cai |
1.北京石油化工学院化学工程学院;2.中国科学院过程工程研究所生化工程国家重点实验室 |
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| Abstract: |
| A method was explored for the preparation of affinity chromatographic support， based on the hydrophilic gigaporous polymer microspheres using Protein A as affinity ligands.The effect of H5IO6 on amount of Protein A was evaluated.The morphology of the microspheres was observed by scanning electronic microscopy.The result showed that the throughput pores were maintained in the affinity media.At different flow rates(361-3 600 cm/h)，the dynamic capacity of human Ig G on the affinity supports were determined,and the result indicated the capacity at 3 600 cm/h decreased about 10% in comparison with that at 361 cm/h.The above results indicated that this affinity support could meet the requirement for rapid and high-throughput separation of proteins. |
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