Rapid and Simultaneous Determination of Seven Active Components in Compound Salvia Miltiorrhiza Bunge Tablets by Flow Rate/Organic Solvent Double-gradient High Performance Liquid Chromatography

KeyWord:flow rate/organic solvent double-gradient  monolithic column  high performance liquid chromatography(HPLC)  compound Salvia miltiorrhiza bunge tablets  DryLab

Abstract:

      A flow rate/organic solvent double-gradient mode in reversed phase high performance liquid chromatography(HPLC) by using monolithic column was established as a new approach for the rapid and simultaneous determination of notoginsenoide R1,ginsenosides Rg1,Re,Rb1,Rd,Rb2 and tanshinone ¢òA in compound Salvia miltiorrhiza bunge tablets.Through the optimization of the flow rate/organic solvent double-gradient mode,and combination with chromatographic simulation software DryLab,the optimized double-gradient HPLC system for the seven active components was built.The determination was carried out on a Merck Chromolith Performance RP-18e(100 mm¡Á4.6 mm) column using acetonitrile-water as mobile phase at a column temperature of 30 ¡æ.The separation of seven active components in the tablets was achieved in 21 min.The linear ranges were 60.60-242.40 mg/L(r=0.999 0) for Rg1,16.52-66.08 mg/L(r=0.999 9) for tanshinone ¢òA£¬and 30.70-142.66 mg/L(r¡Ý0.999 4) for the other active components.The LOQs( S/N=10) were in the range of 21-124 ¦Ìg/kg and the mean recoveries were 96.7%-100.1%.The method could be used for the simultaneous determination of different polarity compounds in a short time.It also could improve the column efficiency of the analytes,compress the half-peak width and reduce the trailing.Therefore,the method could be used for the rapid and simultaneous determination of the seven active components in compound Salvia miltiorrhiza bunge tablets.