Determination of 5 Resin Acids in Food Contact Adhesives by HPLC

KeyWord:food package  adhesive  resin acids  high performance liquid chromatography(HPLC)

Abstract:

      A high performance liquid chromatographic(HPLC) method was developed for the determination of 5 resin acids,including abietic acid,neoabietic acid,dehydroabietic acid,palustric acid and levopimaric acid in food contact adhesives£®The conditions of sample pretreatment and chromatographic separation were optimized.The sample was dissolved with ethyl acetate,and the polymer was precipitated with methanol.After filtration with filter membrane,the extract was separated on a Venusil MP C18(2) column with acetonitrile-0.4%acetic acid(80¡Ã20) as mobile phase at a flow rate of 1.0 mL/min,detected accurately by HPLC with diode-array detector(DAD) at 240 nm for abietic acid and neoabietic acid,270 nm for palustricacid and levopimaric acid,208 nm for dehydroabietic acid,and quantified by the external standard method£®The HPLC method showed a good linearity above two orders of magnitude£¬with detection limits of 2.5-10.0 mg/kg.The RSDs for 5 resin acids were no more than 3.4%,and the recoveries were in the range of 92.2%-103.6%£®The method has the advantages of simple operation,rapid analysis and less interference factors,and could provide credible analysis for 5 resin acidsin food contact adhesives.