Simultaneous Determination of Bisdemethoxycurcumin£¬Demethoxycurcumin and Curcumin in Fish Tissues by UPLC

KeyWord:fish tissues  bisdemethoxycurcumin  demethoxycurcumin  curcumin  ultra high performance liquid chromatography£¨UPLC£©

Abstract:

      An original method for the simultaneous determination of bisdemethoxycurcumin£¬demethoxycurcumin and curcumin in plasma£¬muscle£¬liver and kidney of yellow catfish£¨Pelteobagrus fulvidraco£©£¬bluntnose black bream£¨Megalobrama amblycephala£© and grass carp£¨Ctenopharyngodon idellus£© was established by ultra high performance liquid chromatography with ultraviolet detection£¨UPLC-UV£©.The sample was extracted using acetonitrile containing 0.01% acetic acid£¬and the moisture of sample was removed with anhydrous sodium sulfate£¬then n-hexane was used for removing fat in sample.After that£¬the sample prepared was separated on an ACQUIT UPLC BEH C18 column and determined at 428 nm.Good linearities were observed in the range of 0.01-5.00 mg/L£¬and the correlation coefficients for bisdemethoxycurcumin£¬demethoxycurcumin and curcumin were 0.998 7£¬0.999 8 and 0.999 6£¬respectively.The average recoveries of 3 analytes in fish tissues at the spiked level of 0.025-1.00 mg/kg ranged from 64.7% to 102.2%,with relative standard deviations£¨RSDs£© of 0.69%-10.8%.The detection limits£¨LODs£© and quantitation limits£¨LOQs£© for 3 analytes in fish tissues were all 0.010 mg/kg and 0.025 mg/kg£¬respectively.The presented method was firstly applied in the investigation on the pharmacokinetic profile of curcumin in bluntnose black bream.