Determination of Acrylamide in Rice Product by Matrix Solid Phase Dispersion£¨MSPD£©-Capillary Electrophoresis

KeyWord:acrylamide  matrix solid-phase dispersion(MSPD)  capillary electrophoresis  rice product

Abstract:

      A new method coupling matrix solid phase dispersion£¨MSPD£© with capillary electrophoresis was developed for the determination of acrylamide in rice product.The optimized conditions obtained in experiments were as follows£ºFlorisil and C18 were used as a mixed dispersant with a mass ratio of 1¡Ã4.The mass ratio of sample to dispersant was 5¡Ã9.The mixture of 10 mL acetone-dichloromethane£¨6¡Ã4£© was used as elution solvent.Under the optimized experimental conditions£¬20 mmol/L borate solution£¨pH 8.4£© was used as buffer.The sample was separated at a constant voltage of 15 kV.The linear range of acrylamide was in the range of 10-200 ¦Ìg/mL£¬with a correlation coefficient£¨r£© of 0.999 8.The limit of detection£¨LOD£¬S/N=3£© was 0.62 ¦Ìg/mL for acrylamide and the recoveries were in the range of 85.7%-96.4%.