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Application of Ultra Performance Liquid Chromatography-Quadrupole Time-of-flight Mass Spectrometry in Determination of CML and CEL in Baking Foods |
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DOI: |
KeyWord:ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS) baking foods Nε-(carboxymethyl)lysine(CML) Nε-(carboxyethyl)lysine(CEL) |
Author | Institution |
LIN Qin,ZHENG Xiao-yan,CHEN Chun,DAI Ming,ZHOU Yan-qiong,ZHANG Ying |
1.福建省产品质量检验研究院;2.浙江大学 生物系统工程与食品科学学院 |
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Hits: 2006 |
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Abstract: |
A method was developed for the determination of Nε-(carboxymethyl)lysine(CML) and Nε-(carboxyethyl)lysine(CEL) in baking food using ultra performance liquid chromatography-quadrupole Time-of-flight mass spectrometry(UPLC-Q-TOF/MS).CML and CEL were released from the baking foods through the process of sample degreasing, reduction reaction,precipitation and acid hydrolysis of acquired protein,treated by pre-column derivation with FMOC-Cl,and purified with HLB solid-phase extraction(SPE) column.Then the contents of CML and CEL in pretreated samples were determined by UPLC-Q-TOF/MS. All the target compounds exhibited good linearity(r>0.999) over the concentration range of 1-700ng/mL.The detection limits of CML and CEL were all 0.1mg/kg and the mean recoveries were in the range of 94.4%-108.3% with relative standard deviations(RSD,n=6) of 0.4%-6.2%.The method was accurate and sensitive,and was suitable for the detection of CML and CEL contents in baking foods. |
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