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Rapid Determination of Diclazuril,Toltrazuril and Their Metabolite Residues in Foods of Animal Origin by On line Cleanup Liquid Chromatography-High Resolution Mass Spectrometry |
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DOI: |
KeyWord:on-line cleanup obitrap high resolution mass spectrometry diclazuril toltrazuril toltrazurilsulfone toltrazuril sulfoxide foods of animal origin |
Author | Institution |
ZHANG Hai-chao,MA Yu-song,LI Ying,AI Lian-feng,XU Niu-sheng,CHEN Rui-chun,GUO Chun-hai |
1.河北出入境检验检疫局;2.赛默飞世尔科技(中国)有限公司 |
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Abstract: |
A new method was developed for the rapid determination of diclazuril,toltrazuril,toltrazurilsulfone and toltrazurilsulfoxide residues in foods of animal origin by on line cleanup liquid chromatography-high resolution benchtop quadropole/obitrap mass spectrometry(HRMS) .The samples were extracted with acetonitrile,and purified on a Cyclone column.The analytes were eluted from the exaction column and put into a C18 analytical column prior to chromatographic separation and detection in full scan/ddMS2 mode.The calibration curves were linear in the range of 0.5-50 ng/mL with correlation coefficients more than 0.999 4.The limits of quantitation(LOQ) were 2 μg/kg.Average recoveries of the analytes in four matrices ranged from 80.2% to 110.5%,with relative standard deviations of 2.8%-9.7%.With simple pretreatment,low matrix effects,this method could be used for the rapid confirmation with accurate mass of quasi molecular ion,retention time,isotope ratio and qualitative fragments. |
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