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| Rapid and Simultaneous Determination of 11 Penicillin Residues in Fishery Products by Pass through SPE Purification and Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:penicillins pass through solid phase extraction liquid chromatography tandem mass spectrometry（LC-MS/MS） fishery products |
| Author | Institution |
| GUO Meng-meng，LI Zhao-xin*，WANG Zhi，PAN Ming-xuan，WU Hai-yan，XING Li-hong，SUN Xiao-jie |
1.中国水产科学研究院黄海水产研究所农业部水产品质量安全检测与评价重点实验室;2.青岛市产品质量监督检验研究院青岛市产品质量检验技术研究所;3.上海海洋大学 |
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| Abstract: |
| A method was developed for the rapid and simultaneous determination of 11 penicillins in fishery products by pass-through SPE purification with liquid chromatography tandem mass spectrometry（LC-MS/MS）．The samples were extracted with acetonitrile-water（80∶20，by volume），and then cleaned up with an Oasis PRiME HLB pass through SPE column.The separation of 11 penicillins were performed on a Kinetex XB-C18（2.1 mm×100 mm，2.6 μm） column by using a mixture of 0.05% formic acid and acetonitrile containing 0.05% formic acid as mobile phase.Qualitative and quantitative analyses of the analyte were carried out under the multiple reaction monitoring（MRM） mode with positive electrospray ionization．And the isotope internal standards were employed for the quantification.The calibration curves were linear well in corresponding concentration ranges,with correlation coefficient over 0.99.The detection limits ranged from 0.30 μg/kg to 1.5 μg/kg.The average spiked recoveries for 11 penicillins were between 85.5%and 110% with relative standard deviations（RSDs） of 5.9%-14.3%．The proposed method is accurate，sensitive and high-efficient，and is practical for the determination of penicillins in fishery products． |
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