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Determination of Azithromycin in Formula Feed for Fattening Pigs by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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DOI: |
KeyWord:liquid chromatography tandem mass spectrometry(LC-MS/MS) azithromycin fattening pig formula feed |
Author | Institution |
TANG Xiang,ZHAO Li-jun,LUO Cheng,WU Cai-mei*,YAN Shan-shan,LIU Guang-mang,LIN Yan,WU De |
1.四川农业大学动物营养研究所,四川省动物抗病营养重点实验室,农业部动物抗病营养与饲料重点实验室;2.农业部畜禽产品质量安全风险评估实验室(成都);3.四川大学化学工程学院 |
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Abstract: |
A method was developed for the determination of azithromycin in formula feed for fattening pigs by liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The sample was extracted with acetonitrile under ultrasonic assistance.Oasis MCX solid phase extraction column was used for purification,and the chromatographic separation was performed on a Hypersil BDS C18 column(2.4 μm,100 mm×2.1 mm) with gradient elution using 0.05 mol/L ammonium acetate-methanol(1∶9) as mobile phase.The detection of azithromycin was performed with positive ion under multiple reaction monitoring(MRM) mode.The internal standard isotope dilution method was used for quantification.A good linearity(r2=0.999 1) was obtained for azithromycin in the range of 1-250 μg/L.The limit of detection(LOD,S/N≥3) and the limit of quantitation(LOQ,S/N≥10) of azithromycin in the formula feed were 2.8 μg/kg and 8.4 μg/kg,respectively.Average recoveries ranged from 87.0% to 106.6% for the formula feed at spike levels of 0.5,1,5,10 mg/kg.The intra-RSDs for azithromycin were 0.58%-5.4% and the inter-RSDs were 3.1%-5.4%.The method is accurate and sensitive,and the matrix interference is small.The method could be applied in the determination of azithromycin in formula feed. |
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