|
Determination of Pb2+ and Ni2+ in Soy Sauce by Online Derivation with High Performance Liquid Chromatography |
|
View Full Text Download reader |
DOI: |
KeyWord:high performance liquid chromatography(HPLC) online derivation soy sauce heavy metal ions |
Author | Institution |
LU Yu-xi,ZHANG Li-hong,TANG Li-li,ZHANG Shuang,LUAN Feng,LIU Hui-tao |
烟台大学化学化工学院 |
|
Hits: 2460 |
Download times: 848 |
Abstract: |
Contents of Pb2+ and Ni2+ in soy sauce were determined by the online derivation and HPLC method with wet digestion.Sodium diethyldithiocarbamate(NaDDTC) was used as the derivtive agent for heavy metal ions.The experimental conditions were as follows:chromatography column:a Hypersil ODS2 C-18(5 μm,250 mm×4.6 mm) column,mobile phase:MeOH-H2O-sodium diethyldithiocarbamate(63.5∶35∶1.5).The calibration curves for Pb2+ and Ni2+ were linear in the range of 0.5-50 μg/mL with correlation coefficients of 0.998 2 and 0.999 0,respectively.The limits of detection were 0.3 μg/mL and 0.2 μg/mL,respectively,with recoveries of 88.1%-91.8%.The method has the advantages of simplicity,good accuracy and precision,and could be used as an alternative method for the determination of heavy metal ions in real soy sauce samples. |
Close |