Quantitation of Squalene in Chinese Liquor Using Multistage Liquid-Liquid Microextraction Coupled with Gas Chromatography-Mass Spectrometry

KeyWord:squalene  Chinese liquor  multistage LLME  GC-MS  quantitation analysis

Abstract:

      A multistage liquid-liquid microextraction(LLME) coupled with GC-MS method was developed to determine squalene in Chinese liquor.After diluting the liquor sample,a multistage microextraction was carried out using pentane-diethyl ether mixture(1¡Ã3,by volume),then the organic phase was concentrated with nitrogen,and analyzed by GC-MS.The result indicated that squalene had a good linearity in the range of 0.62-159.88 ¦Ìg/L,with a correlation coefficient of 0.995 5.The detection limit of squalene was as low as 0.17 ¦Ìg/L.The average recoveries of squalene at spiked levels of 2,20,40 ¦Ìg/L were 78.1%,85.0%,103.5%,respectively,with relative deviations of 12.1%,8.9%,7.0%,respectively.With the advantages of simplicity,accuracy,high-extraction efficiency and low consumption of organic solvent,this method was suitable for the quantitative analysis of squalene in Chinese liquor.