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Determination of Amitraz,Chlordimeform and Their Metabolites in Blood by Solid Supported Liquid Extraction and Gas Chromatography-Tandem Mass Spectrometry |
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DOI: |
KeyWord:gas chromatography-tandem mass spectrometry(GC-MS/MS) solid supported liquid extraction blood chlordimeform amitraz metabolites |
Author | Institution |
GAO Xue,LIU Wen-wen,WANG Xing-min |
1.重庆工商大学天然药物研究重庆市重点实验室;2.重庆工商大学 环境与资源学院;3.重庆市公安局物证鉴定中心 |
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Abstract: |
A method was developed for the determination of amitraz,chlordimeform and their metabolites in blood by gas chromatography-tandem mass spectrometry(GC-MS/MS).The conditions for sample pretreatment and GC-MS/MS parameters were optimized.The samples were pretreated by solid supported liquid extraction(SLE),and eluted with acetonitrile-dichloromethane(1∶1).The detection was carried out by GC-MS/MS in multiple reaction monitoring(MRM) mode.Good linear relationships for 2,4-dimethylaniline(DMA),4-chloro-2-methylaniline and amitraz were obtained in the range of 1.0-1 000 ng/mL,and those for other three compounds were obtained in the range of 2.0-1 000 ng/mL,with correlation coefficients more than 0.99.The limits of quantitation were in the range of 1.0-2.0 ng/mL,and the recoveries ranged from 88.6% to 113%.With the advantages of simplicity,rapidness,low sample consumption,accuracy and reliability,this methed is suitable for the determination of six compounds in blood. |
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